Mitgo

Mitgo


The biosensors for mitochondrial function described here are both based on GFP. Removal of the excitation filter allows excitation at both nm and nm without the need to switch filters, thus increasing achievable time resolution. Such segregation could contribute to mother-daughter age asymmetry and the rejuvenation of daughter cells. Illumination at nm optimally excites the oxidized species of roGFP. Interestingly, our preliminary data indicates that there may be differences in ATP levels in different mitochondria within the same cell Figure 6D. We used roGFP1 to probe the redox status of mitochondria in the context of aging of the model fungus Saccharomyces cerevisiae.

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Mitgo


The biosensors for mitochondrial function described here are both based on GFP. Removal of the excitation filter allows excitation at both nm and nm without the need to switch filters, thus increasing achievable time resolution. Such segregation could contribute to mother-daughter age asymmetry and the rejuvenation of daughter cells. Illumination at nm optimally excites the oxidized species of roGFP. Interestingly, our preliminary data indicates that there may be differences in ATP levels in different mitochondria within the same cell Figure 6D. We used roGFP1 to probe the redox status of mitochondria in the context of aging of the model fungus Saccharomyces cerevisiae.

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